Experimental Pharmacology & Analytical Biochemistry

Experimental Pharmacology

Analytical Biochemistry

This unit supplies biochemical and cell-based screenings aimed to assess the biological activity and toxicity profile of small molecules and/or biologics.
The custom cell-based assays and the high-throughput screening (HTS) platforms allow to rapidly characterize the test compound in terms of mechanism of action, cytotoxicity and other properties.

Experimental Activities

	Cell culture (maintenance and storage, tests for pathogens and toxins identifications)
	Isolation of new primary cell lines
	Cytotoxicity and proliferation assays
	XC50 testing and confirmations
	Signal transduction assays
	Reporter gene assays
	Cytokine release assays
	FACS
	Chemotaxis, migration and angiogenesis
	Live Cell Imaging (Operetta High-Content Imaging System)
	Evaluation of ligand-receptor affinity by SPR (Biacore T200)
	Immunoassays (ELISA, Western blot, Immunofluorescence)
On the basis of the experimental requirements it is possible to choose between more than 100 cellular models, including:
	stabilized cell lines
	primary cells from different tumour types
	unique pre-clinical models of malignant mesothelioma
	engineered cell lines expressing GFP or Luciferase reporter genes